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KMID : 0903519870300040364
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Journal of the Korean Society of Agricultural Chemistry and Biotechnology
1987 Volume.30 No. 4 p.364 ~ p.370
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Postchilling Accumulation of Superoxide in Cells and Chilling Injury in Rice Plant
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Abstract
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The O^-₂ level of the extract from young rice leaves, which was cold treated for 2 days and then placed at room temperature for a period of time significantly higher than that from tissues untreated. O^-₂ level in leaves was practically unchanged during cold treatment for 48 hours. But it started to increase to arrive at maximum in 8 hours, once the plants were placed under room temperature. The abnormal production of O^-₂ in mitochondria during postchilling process was interpreted as a biochemical consequence of accumulation of glycolysis product(s) in cytosol and/or NADH in mitocisondrial matrix due to disruption of catabolic balance at low temperature, Mitochondria isolated from the chilling injured tissue was found to have lost considerably their respiratory activity. This fact may imply the involvement of intramitochondrial accumulation of O^-₂ in the inactivation of electron transport chain system. The observation that mitochondria in the presence of the O^-₂-producing enzymatic system (Xanthine/Xanthine oxidase) lost their respiratory activity supports this inference. It was also found in this work that Superoxide dismutase (SOD) is a substrate inducible enzyme, and that SOD is a possible protective agent in plant cell against chilling injury.
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